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NEB and Mammoth Biosciences launch MB-CasFire enzyme for one-pot isothermal assays
NEB will manufacture and globally distribute Mammoth Biosciences' proprietary MB-CasFire enzyme, a thermostable Type V CRISPR nuclease that works across 37-65 °C and can be coupled directly with LAMP, RPA and NEAR assays. MB-CasFire resolves targets at single-nucleotide resolution and simplifies assay architecture by eliminating the need for multiple temperature incubations or component additions.